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Antibody Product Overview
| ELK.No | EM1003 |
| Product name | His-Tag Mouse mAb |
| Reactivity | |
| Applications | WB, IP, IF |
| Other name | |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | |
| Human Swiss-Prot | |
| Source | Mouse |
| Isotype | IgG1 |
| Target | |
| Fields | |
| Gene name | |
| Protein name | |
| Human gene link | |
| Human Swiss link | |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthetic Peptide |
| Specificity | |
| Formulation | |
| Clonality | Monoclonal |
| Dilution | WB 1:5,000 IP 1:200 IF 1:1,000 |
| Purification | |
| Concentration | 1mg/mL |
| Storage stability | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Molecular Weight (Da) | |
| Observed band (KD) | |
| Background | A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. |
| Function | |
| Subcellular location | |
| Expression |
Additional Images
IP antibody use：5ug His Mouse IgG1 per ml Lysate，WB 1:3000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with His-tag fusion protein 3、IP (untransfected 293+anti-His mAb+ Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Prote
IF analysis of 293 cells transfected with a His-tag protein, using ELKbio anti-HisTag Mouse mAb at a 1:1000 dilution (blue DAPI, red anti-His)
2ug His fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000
1、Multi-Tag Recombinant protein 2、293T transfected with His recombinant protein 3、Insect cell trandfected with His recombinant protein
: EM1003-50
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