Product Information
|
Product Name |
Cat. No. |
Spec. |
|
Coomassie Brilliant Blue Ultrafast Staining |
AO-03-G2059-250ML |
250 mL |
|
AO-03-G2059-500ML |
500 mL |
Product Description/Introduction
Coomassie Brilliant Blue Ultrafast Staining solution is a staining solution with Coomassie brilliant blue G250 as the dye. It can be used to stain protein gels such as SDS-PAGE or non-denaturing PAGE, and can also be used to detect residual proteins on PAGE gels after Western Blot. The staining solution is free of methanol and acetic acid, which are toxic in conventional stains; it is pollution-free, non-toxic, non-irritating odor, highly environmental protection; No fixation, shorter staining time and high sensitivity with this stain; 50 ng of protein can be detected in half an hour for a 1.0 mm thick gel; 20 ng of protein can be detected in as little as 1 hour of staining. After staining, protein bands can be observed with or without washing for 30 min.
Storage and Shipping Conditions
Ship at room temperature; Store in the dark at 2-8℃, valid for 12 months.
Product Components
|
Component |
AO-03-G2059-250ML |
AO-03-G2059-500ML |
|
Coomassie Bright Blue Ultrafast Staining |
250 mL |
500 mL |
|
Manual |
||
Assay Protocol/Procedures
1. Remove the polyacrylamide gel after electrophoresis, put it into a clean vessel, clean it with pure water three times, 20 s each time；
2. Pour out pure water, add 20 mL of Coomassie brilliant blue ultrafast staining solution (to submerge the gel), and shake for 30 min (the gel staining time can be adjusted in real time according to the depth of the protein bands)；
3. Pour out the staining solution and wash with pure water by shaking for 30 min. The longer the decolorization time, the lighter the background will be (adjust the decolorization time according to the experimental needs). If the gel with lower background is needed, the gel can be decolorized with pure water for more than 1 h or overnight.
Note
1. The stain will settle at the bottom of the bottle after a period of time. Please mix it upside down before use.
2. The staining solution is acidic and slightly corrosive. Please take protective measures.
3. Cleaning with pure water before dyeing can greatly reduce the interference of SDS.
4. Take photos quickly after dyeing. Long immersion in pure water will lead to the loss of protein and dye.
5. For your safety and health, please wear safety glasses, gloves, or protective clothing.
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