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EasyStep Human aFP(Alpha-Fetoprotein) ELISA Kit
AO-06-ELK091ES-48T
Product Overview
| Cat.No | ELK091ES |
| Product Name | EasyStep Human aFP(Alpha-Fetoprotein) ELISA Kit |
| Reactivity | Human |
| Alternative Names | A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein |
| Assay Type | Sandwich |
| Sensitivity | 0.175 ng/mL |
| Standard | 100 ng/mL |
| Detection Range | 1.56-100 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Tumor immunity;Hepatology; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human aFP, and the Human aFP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human aFP. After TMB substrate solution is added, only those wells that contain Human aFP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aFP in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK091ES-48T
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