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Antibody Product Overview
| ELK.No | ES1000 |
| Product name | Acrosin rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | ACR; ACRS; Acrosin |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 49 |
| Human Swiss-Prot | P10323 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Acrosin |
| Fields | |
| Gene name | ACR |
| Protein name | Acrosin |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P23578 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized peptide derived from Acrosin . at AA range: 40-120 |
| Specificity | Acrosin Polyclonal Antibody detects endogenous levels of Acrosin protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 45kD |
| Background | Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.,function:Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.,similarity:Belongs to the peptidase S1 family.,similarity:Contains 1 peptidase S1 domain.,subunit:Heavy chain (catalytic) and a light chain linked by two disulfide bonds., |
| Subcellular location | acrosomal vesicle,extracellular region,nucleus,Golgi-associated vesicle,acrosomal matrix,protein complex, |
| Expression | Leukocyte,Testis, |
Additional Images
Western Blot analysis of various cells using Acrosin Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
: AO-06-ES1000-50
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