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Cleaved-MMP-14 (Y112) rabbit pAb
AO-06-ES1044-50
Antibody Product Overview
| ELK.No | ES1044 |
| Product name | Cleaved-MMP-14 (Y112) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | MMP14; Matrix metalloproteinase-14; MMP-14; MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 4323 |
| Human Swiss-Prot | P50281 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MMP-14 |
| Fields | >>TNF signaling pathway;>>GnRH signaling pathway;>>Parathyroid hormone synthesis, secretion and action |
| Gene name | MMP14 |
| Protein name | Matrix metalloproteinase-14 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 17387 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P53690 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 81707 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q10739 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MMP14. AA range:93-142 |
| Specificity | Cleaved-MMP-14 (Y112) Polyclonal Antibody detects endogenous levels of fragment of activated MMP-14 protein resulting from cleavage adjacent to Y112. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 53kD |
| Background | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.,cofactor:Binds 1 zinc ion per subunit.,cofactor:Calcium.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,function:Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.,PTM:The precursor is cleaved by a furin endopeptidase.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 4 hemopexin-like domains.,s |
| Subcellular location | Membrane ; Single-pass type I membrane protein . Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm. |
| Expression | Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. |
Additional Images
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Western Blot analysis of various cells using Cleaved-MMP-14 (Y112) Polyclonal Antibody
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Western blot analysis of lysates from 293 cells, treated with etoposide 25uM 1h, using MMP14 (Cleaved-Tyr112) Antibody. The lane on the right is blocked with the synthesized peptide.
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: AO-06-ES1044-50
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