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Antibody Product Overview
| ELK.No | ES11787 |
| Product name | CD8B rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 926 |
| Human Swiss-Prot | P10966 |
| Source | Rabbit |
| Isotype | IgG |
| Target | CD8B |
| Fields | >>Cell adhesion molecules;>>Antigen processing and presentation;>>Hematopoietic cell lineage;>>T cell receptor signaling pathway;>>Yersinia infection;>>Primary immunodeficiency |
| Gene name | CD8B CD8B1 |
| Protein name | T-cell surface glycoprotein CD8 beta chain (CD antigen CD8b) |
| Human gene link | |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P10300 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | P05541 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from part region of human protein |
| Specificity | CD8B Polyclonal Antibody detects endogenous levels of protein. |
| Formulation | Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000 ELISA 1:5000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 23kD |
| Background | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010], |
| Function | function:Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.,online information:CD8 entry,PTM:Phosphorylated as a consequence of T-cell activation .,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subunit:In general heterodimer of an alpha and a beta chain linked by two disulfide bonds., |
| Subcellular location | [Isoform 1]: Cell membrane ; Single-pass type I membrane protein . Requires the partner CD8A for efficient cell surface expression (PubMed:3145196). The heterodimer CD8A/CD8B localizes to lipid rafts due to CD8B cytoplasmic tail palmitoylation. .; [Isoform 2]: Cell membrane ; Single-pass type I membrane protein .; [Isoform 3]: Secreted .; [Isoform 4]: Cell membrane ; Single-pass type I membrane protein .; [Isoform 5]: Cell membrane ; Single-pass type I membrane protein .; [Isoform 6]: Secreted .; [Isoform 7]: Secreted .; [Isoform 8]: Secreted . |
| Expression | Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. |
Additional Images
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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES11787-50
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