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Antibody Product Overview
| ELK.No | ES12458 |
| Product name | UBA3 rabbit pAb |
| Reactivity | Human; Mouse;Rat |
| Applications | WB |
| Other name | |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 9039 |
| Human Swiss-Prot | Q8TBC4 |
| Source | Rabbit |
| Isotype | IgG |
| Target | UBA3 |
| Fields | >>Ubiquitin mediated proteolysis |
| Gene name | UBA3 UBE1C |
| Protein name | UBA3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 22200 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8C878 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 117553 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q99MI7 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from human UBA3 AA range: 105-155 |
| Specificity | This antibody detects endogenous levels of UBA3 at Human/Mouse/Rat |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1：500-2000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 51kD |
| Observed band (KD) | |
| Background | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008], |
| Function | enzyme regulation:Binding of TP53BP2 to the regulatory subunit NAE1 decreases activity.,function:Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.,miscellaneous:Arg-211 acts as a selectivity gate, preventing misactivation of ubiquitin by this NEDD8-specific E1 complex.,pathway:Protein modification; protein neddylation.,similarity:Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.,subunit:Heterodimer of UBA3 and NAE1. Interacts with NEDD8, UBE2F and UBE2M. Binds ESR1 and ESR2 with bound steroid ligand.,tissue specificity:Ubiquitousl |
| Subcellular location | nucleus,cytosol, |
| Expression | Ubiquitously expressed. |
Additional Images
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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES12458-100
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