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TIF1β (phospho-Ser824) rabbit pAb
AO-06-ES12702-100
Antibody Product Overview
| ELK.No | ES12702 |
| Product name | TIF1β (phospho-Ser824) rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA;IHC |
| Other name | Transcription intermediary factor 1-beta (TIF1-beta) (E3 SUMO-protein ligase TRIM28) (EC 6.3.2.-) (KRAB-associated protein 1) (KAP-1) (KRAB-interacting protein 1) (KRIP-1) (Nuclear corepressor KAP-1) (RING finger protein 96) (Tripartite motif-containing protein 28) |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 10155 |
| Human Swiss-Prot | Q13263 |
| Source | Rabbit |
| Isotype | IgG |
| Target | TIF1β |
| Fields | |
| Gene name | TRIM28 KAP1 RNF96 TIF1B |
| Protein name | TIF1β (Ser824) |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 21849 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q62318 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 116698 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | O08629 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized phosho peptide around human TIF1β (Ser824) |
| Specificity | This antibody detects endogenous levels of Human TIF1β (phospho-Ser824) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 110kD |
| Background | The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008], |
| Function | domain:Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.,domain:The HP1 box is both necessary and sufficient for HP1 binding. The RING finger domain and the B-box domains mediate interaction with CEBPB. The PHD domain enhances the CEBPB transcriptional activity.,function:Forms a complex with a KRAB-domain transcription factor and increases the efficiency of KRAB-mediated repression. Silences transcription through an interaction with HP1 proteins. Acts as a corepressor of transcription for the KRAB zinc finger proteins and as a moderator of the repression activity. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of the Alpha-1-acid glycoprotein gene.,PTM:Phosphorylated upon DNA |
| Subcellular location | Nucleus . Associated with centromeric heterochromatin during cell differentiation through CBX1. . |
| Expression | Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. |
Additional Images
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
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: AO-06-ES12702-100
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