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Calnexin (phospho Ser583) rabbit pAb
AO-06-ES1276-100
Antibody Product Overview
| ELK.No | ES1276 |
| Product name | Calnexin (phospho Ser583) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | CANX; Calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 821 |
| Human Swiss-Prot | P27824 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Calnexin |
| Fields | >>Protein processing in endoplasmic reticulum;>>Phagosome;>>Antigen processing and presentation;>>Thyroid hormone synthesis;>>Human T-cell leukemia virus 1 infection |
| Gene name | CANX |
| Protein name | Calnexin |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 12330 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P35564 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 29144 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P35565 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Calnexin around the phosphorylation site of Ser583. AA range:543-592 |
| Specificity | Phospho-Calnexin (S583) Polyclonal Antibody detects endogenous levels of Calnexin protein only when phosphorylated at S583. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 90kD |
| Background | This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008], |
| Function | function:Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.,online information:Calnexin entry,similarity:Belongs to the calreticulin family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV., |
| Subcellular location | Endoplasmic reticulum membrane ; Single-pass type I membrane protein . Endoplasmic reticulum . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed:22314232). . |
| Expression | B-cell lymphoma,Epithelium,Fibroblast,Keratinocyte,Kidney,Liver,Lymph,Placenta,Platelet, |
Additional Images
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Western Blot analysis of various cells using Phospho-Calnexin (S583) Polyclonal Antibody diluted at 1:2000
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Calnexin (Phospho-Ser583) Antibody
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Immunofluorescence analysis of HeLa cells, using Calnexin (Phospho-Ser583) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Calnexin (Phospho-Ser583) Antibody. The picture on the right is blocked with the phospho peptide.
: AO-06-ES1276-100
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