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Cyclin B1 (phospho Ser126) rabbit pAb
AO-06-ES1294-50
Antibody Product Overview
| ELK.No | ES1294 |
| Product name | Cyclin B1 (phospho Ser126) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | CCNB1; CCNB; G2/mitotic-specific cyclin-B1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 891 |
| Human Swiss-Prot | P14635 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Cyclin B1 |
| Fields | >>FoxO signaling pathway;>>Cell cycle;>>Oocyte meiosis;>>p53 signaling pathway;>>Cellular senescence;>>Progesterone-mediated oocyte maturation;>>Human immunodeficiency virus 1 infection |
| Gene name | CCNB1 |
| Protein name | G2/mitotic-specific cyclin-B1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 268697 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P24860 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25203 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P30277 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cyclin B1 around the phosphorylation site of Ser126. AA range:91-140 |
| Specificity | Phospho-Cyclin B1 (S126) Polyclonal Antibody detects endogenous levels of Cyclin B1 protein only when phosphorylated at S126. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 60kD |
| Background | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008], |
| Function | developmental stage:Accumulates steadily during G2 and is abruptly destroyed at mitosis.,function:Essential for the control of the cell cycle at the G2/M (mitosis) transition.,PTM:Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin AB subfamily.,subunit:Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase., |
| Subcellular location | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
| Expression | Breast adenocarcinoma,Lung,Placenta, |
Additional Images
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Western Blot analysis of various cells using Phospho-Cyclin B1 (S126) Polyclonal Antibody
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Cyclin B1 (Phospho-Ser126) Antibody
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Cyclin B1 (Phospho-Ser126) Antibody. The picture on the right is blocked with the phospho peptide.
: AO-06-ES1294-50
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