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Antibody Product Overview
| ELK.No | ES12999 |
| Product name | SPAST rabbit pAb |
| Reactivity | Human; Mouse;Rat |
| Applications | WB |
| Other name | |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 6683 |
| Human Swiss-Prot | Q9UBP0 |
| Source | Rabbit |
| Isotype | IgG |
| Target | SPAST |
| Fields | |
| Gene name | SPAST ADPSP FSP2 KIAA1083 SPG4 |
| Protein name | SPAST |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 50850 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9QYY8 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 362700 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | B2RYN7 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from human SPAST AA range: 163-213 |
| Specificity | This antibody detects endogenous levels of SPAST at Human/Mouse/Rat |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1：500-2000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 68kD |
| Observed band (KD) | |
| Background | This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The encoded ATPase may be involved in the assembly or function of nuclear protein complexes. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequences have not been determined. Mutations associated with this gene cause the most frequent form of autosomal dominant spastic paraplegia 4. [provided by RefSeq, Jul 2008], |
| Function | alternative products:Alternative promoter usage of a cryptic promoter in exon 1 can direct the synthesis of N-terminally truncated isoforms, which may also arise from alternative initiation,catalytic activity:ATP + H(2)O = ADP + phosphate.,developmental stage:Expressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus.,disease:Defects in SPAST are the cause of spastic paraplegia autosomal dominant type 4 (SPG4) [MIM:182601]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the w |
| Subcellular location | Membrane ; Peripheral membrane protein . Endoplasmic reticulum . Midbody . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton . Cytoplasm, perinuclear region . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasm . Forms an intramembrane hairpin-like structure in the membrane (PubMed:20200447). Localization to the centrosome is independent of microtubules (PubMed:15891913). Localizes to the midbody of dividing cells, and this requires CHMP1B (PubMed:18997780). Enriched in the distal axons and branches of postmitotic neurons (PubMed:15269182). .; [Isoform 1]: Endoplasmic reticulum membrane ; Peripheral membrane protein . Nucleus membrane . Lipid droplet . Cytoplasm, cytoskeleton . Endosome . Forms an intramembrane hairpin-like structure in the mem |
| Expression | Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. The short isoforms may predominate in brain and spinal cord. |
Additional Images
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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES12999-50
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