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Antibody Product Overview
| ELK.No | ES13100 |
| Product name | SIN1 rabbit pAb |
| Reactivity | Human; Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 79109 |
| Human Swiss-Prot | Q9BPZ7 |
| Source | Rabbit |
| Isotype | IgG |
| Target | SIN1 |
| Fields | >>mTOR signaling pathway |
| Gene name | MAPKAP1 MIP1 SIN1 |
| Protein name | SIN1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 227743 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8BKH7 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 296648 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q6AYF1 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from human SIN1 AA range: 193-243 |
| Specificity | This antibody detects endogenous levels of SIN1 at Human/Mouse/Rat |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 57kD |
| Observed band (KD) | |
| Background | This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq, Jul 2008], |
| Function | function:Essential component of the TORC2 complex, which plays a critical role in AKT1 'Ser-473' phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. Inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAs signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.,similarity:Belongs to the SIN1 family.,subunit:All isoforms except isoform 4 are able to be part of the target of rapamycin 2 complex (TORC2) comprised of FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2.,tissue specificity:Ubiquitously expressed, with highest levels in heart and skeletal muscle., |
| Subcellular location | Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle. Nucleus. |
| Expression | Ubiquitously expressed, with highest levels in heart and skeletal muscle. |
Additional Images
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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES13100-100
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