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Antibody Product Overview
| ELK.No | ES13396 |
| Product name | RIFK rabbit pAb |
| Reactivity | Human; Mouse |
| Applications | WB;ELISA;IHC |
| Other name | |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 55312 |
| Human Swiss-Prot | Q969G6 |
| Source | Rabbit |
| Isotype | IgG |
| Target | RIFK |
| Fields | >>Riboflavin metabolism;>>Metabolic pathways;>>Biosynthesis of cofactors |
| Gene name | RFK |
| Protein name | RIFK |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 54391 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8CFV9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized peptide derived from human RIFK AA range: 82-132 |
| Specificity | This antibody detects endogenous levels of RIFK at Human/Mouse |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 17kD |
| Observed band (KD) | |
| Background | Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009], |
| Function | catalytic activity:ATP + riboflavin = ADP + FMN.,cofactor:Zinc or magnesium.,function:Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).,pathway:Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.,subunit:Monomer.,tissue specificity:Detected in brain, placenta and urinary bladder., |
| Subcellular location | Cytoplasm . |
| Expression | Detected in brain, placenta and urinary bladder. |
Additional Images
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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
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Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES13396-50
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