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PRAS40 (phospho-Ser183) rabbit pAb
AO-06-ES13968-50
Antibody Product Overview
| ELK.No | ES13968 |
| Product name | PRAS40 (phospho-Ser183) rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA;IHC |
| Other name | Proline-rich AKT1 substrate 1 (40 kDa proline-rich AKT substrate) |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 84335 |
| Human Swiss-Prot | Q96B36 |
| Source | Rabbit |
| Isotype | IgG |
| Target | PRAS40 |
| Fields | >>Autophagy - animal;>>mTOR signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Thermogenesis;>>Shigellosis |
| Gene name | AKT1S1 PRAS40 |
| Protein name | PRAS40 (Ser183) |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 67605 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9D1F4 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized phosho peptide around human PRAS40 (Ser183) |
| Specificity | This antibody detects endogenous levels of Human Mouse PRAS40 (phospho-Ser183) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 40kD |
| Background | AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008], |
| Function | function:May play an important role in phosphatidylinositol 3-kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.,subcellular location:Found in the cytosolic fraction of the brain.,subunit:The phosphorylated form interacts with 14-3-3.,tissue specificity:Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293)., |
| Subcellular location | Cytoplasm, cytosol . Found in the cytosolic fraction of the brain. . |
| Expression | Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293). |
Additional Images
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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES13968-50
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