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mTOR (phospho Thr2446) rabbit pAb
AO-06-ES1436-100
Antibody Product Overview
| ELK.No | ES1436 |
| Product name | mTOR (phospho Thr2446) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic tar |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 2475 |
| Human Swiss-Prot | P42345 |
| Source | Rabbit |
| Isotype | IgG |
| Target | mTOR |
| Fields | >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>ErbB signaling pathway;>>HIF-1 signaling pathway;>>Phospholipase D signaling pathway;>>Autophagy - other;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Apelin signaling pathway;>>Neutrophil extracellular trap formation;>>JAK-STAT signaling pathway;>>Th17 cell differentiation;>>Thermogenesis;>>Insulin signaling pathway;>>Thyroid hormone signaling pathway;>>Adipocytokine signaling pathway;>>Type II diabetes mellitus;>>Insulin resistance;>>Growth hormone synthesis, secretion and action;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Kaposi sarcoma-associated herpesvirus infection;>>He |
| Gene name | MTOR |
| Protein name | Serine/threonine-protein kinase mTOR |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 56717 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9JLN9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 56718 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P42346 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human mTOR around the phosphorylation site of Thr2446. AA range:2412-2461 |
| Specificity | Phospho-mTOR (T2446) Polyclonal Antibody detects endogenous levels of mTOR protein only when phosphorylated at T2446. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 220kD |
| Background | The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008], |
| Function | function:Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.,similarity:Belongs to the PI3/PI4-kinase family.,similarity:Contains 1 FAT domain.,similarity:Contains 1 FATC domain.,similarity:Contains 1 PI3K/PI4K domain.,similarity:Contains 7 HEAT repeats.,subunit:Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.,tissue specificity:Expressed in numerous tissues, with highest levels in testis., |
| Subcellular location | Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome . Cytoplasm . Nucleus, PML body . Microsome membrane . Lysosome membrane . Cytoplasmic vesicle, phagosome . Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB (PubMed:18497260, PubMed:20381137). Lysosome targeting also depends on interaction with MEAK7. Translocates to the lysosome membrane in the presence of TM4SF5 (PubMed:30956113). . |
| Expression | Expressed in numerous tissues, with highest levels in testis. |
Additional Images
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Western Blot analysis of various cells using Phospho-mTOR (T2446) Polyclonal Antibody diluted at 1:1000
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using mTOR (Phospho-Thr2446) Antibody
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Western blot analysis of lysates from NIH/3T3 cells treated with Insulin 0.01U/ml 15', using mTOR (Phospho-Thr2446) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES1436-100
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