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RPA p32 (phospho Ser33) rabbit pAb
AO-06-ES1439-50
Antibody Product Overview
| ELK.No | ES1439 |
| Product name | RPA p32 (phospho Ser33) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC |
| Other name | RPA2; REPA2; RPA32; RPA34; Replication protein A 32 kDa subunit; RP-A p32; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 6118 |
| Human Swiss-Prot | P15927 |
| Source | Rabbit |
| Isotype | IgG |
| Target | RFA2 |
| Fields | >>DNA replication;>>Nucleotide excision repair;>>Mismatch repair;>>Homologous recombination;>>Fanconi anemia pathway |
| Gene name | RPA2 |
| Protein name | Replication protein A 32 kDa subunit |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q62193 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 59102 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q63528 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RFA2 around the phosphorylation site of Ser33. AA range:1-50 |
| Specificity | Phospho-RPA p32 (S33) Polyclonal Antibody detects endogenous levels of RPA p32 protein only when phosphorylated at S33. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 32kD |
| Background | function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN., |
| Function | function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN., |
| Subcellular location | Nucleus . Nucleus, PML body . Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner. . |
| Expression | Kidney,Lung,Muscle, |
Additional Images
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Western Blot analysis of various cells using Phospho-RPA p32 (S33) Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western Blot analysis of MCF-7 cells using Phospho-RPA p32 (S33) Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of lysates from NIH/3T3 cells treated with Adriamycin 0.5ug/ml 24h, using RFA2 (Phospho-Ser33) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES1439-50
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