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Histone H2A.X (phospho Ser139) rabbit pAb
AO-06-ES1326-50
Antibody Product Overview
| ELK.No | ES1326 |
| Product name | Histone H2A.X (phospho Ser139) rabbit pAb |
| Reactivity | Human;Mouse;Rat;Hamster |
| Applications | WB;IHC;IF;ELISA |
| Other name | H2AFX; H2AX; Histone H2A.x; H2a/x |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3014 |
| Human Swiss-Prot | P16104 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Histone H2A.X |
| Fields | >>Necroptosis;>>Neutrophil extracellular trap formation;>>Alcoholism;>>Systemic lupus erythematosus |
| Gene name | H2AFX |
| Protein name | Histone H2A.x,γH2AX |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15270 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P27661 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H2A.X around the phosphorylation site of Ser139. AA range:94-143 |
| Specificity | Phospho-Histone H2A.X (S139) Polyclonal Antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at S139. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 15 19kD |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015], |
| Function | developmental stage:Synthesized in G1 as well as in S-phase.,domain:The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.,function:Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.,PTM:Mon |
| Subcellular location | Nucleus . Chromosome . |
| Expression | Lung,Placenta, |
Additional Images
No image
Western blot analysis of lysates from 1) 4T1, 2) 293 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) (Immunoway:YM3028) antibody was diluted at 1:5000 as loading control, 4° over night,Dylight 680 secondary antibody(Immunoway:RS23710)was diluted at 1:10000, 37° 1hour.
No image
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
No image
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
No image
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
: AO-06-ES1326-50
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