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Histone H3 (phospho Ser28) rabbit pAb
AO-06-ES1328-100
Antibody Product Overview
| ELK.No | ES1328 |
| Product name | Histone H3 (phospho Ser28) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968 |
| Human Swiss-Prot | P68431/Q71DI3/P84243 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Histone H3 |
| Fields | >>Neutrophil extracellular trap formation;>>Alcoholism;>>Shigellosis;>>Transcriptional misregulation in cancer;>>Systemic lupus erythematosus |
| Gene name | HIST1H3A |
| Protein name | Histone H3.1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 319152/15077/15078 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | 291159/100361558 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q6LED0/P84245 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H3 around the phosphorylation site of Ser28. AA range:13-62 |
| Specificity | Phospho-Histone H3 (S28) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when phosphorylated at S28. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 20kD |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015], |
| Function | caution:Was originally (PubMed:2587222) thought to originate from mouse.,developmental stage:Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.,function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,mass spectrometry:Monoisotopic with N-acetylserine PubMed:16457589,miscellaneous:This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).,PTM:Acetylation is generally l |
| Subcellular location | Nucleus. Chromosome. |
| Expression | Blood,Epithelium,Kidney,Lung,Ovary,Spleen,Uterus, |
Additional Images
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Western Blot analysis of various cells using Phospho-Histone H3 (S28) Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western Blot analysis of 293T cells using Phospho-Histone H3 (S28) Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of lysates from HUVEC cells treated with Serum 20% 30', using Histone H3 (Phospho-Ser28) Antibody. The lane on the right is blocked with the phospho peptide.
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