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Antibody Product Overview
| ELK.No | ES14446 |
| Product name | NSUN2 rabbit pAb |
| Reactivity | Human; Mouse |
| Applications | WB |
| Other name | tRNA (cytosine(34)-C(5))-methyltransferase (EC 2.1.1.203) (Myc-induced SUN domain-containing protein) (Misu) (NOL1/NOP2/Sun domain family member 2) (Substrate of AIM1/Aurora kinase B) (tRNA (cytosine-5-)-methyltransferase) (tRNA methyltransferase 4 homolog) (hTrm4) |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 54888 |
| Human Swiss-Prot | Q08J23 |
| Source | Rabbit |
| Isotype | IgG |
| Target | NSUN2 |
| Fields | |
| Gene name | NSUN2 SAKI TRM4 |
| Protein name | NSUN2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 28114 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q1HFZ0 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized peptide derived from human NSUN2 AA range: 658-708 |
| Specificity | This antibody detects endogenous levels of NSUN2 at Human/Mouse |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.321% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 85kD |
| Background | This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011], |
| Function | catalytic activity:S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylcytosine.,function:RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation.,PTM:Phosphorylated at Ser-139 by Aurora-B/STK12 during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1.,similarity:Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.,subcellular location:Concentrated in the nucleolus during interphase and distributed in the perichromosome and cytoplasm during mitosis.,subunit:Interacts with NPM1 and NCL during interphase; interaction is disrupted fol |
| Subcellular location | Nucleus, nucleolus . Cytoplasm . Mitochondrion . Cytoplasm, cytoskeleton, spindle . Secreted, extracellular exosome . Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA (PubMed:19596847). In testis, localizes to the chromatoid body (By similarity). . |
| Expression | Expressed in adult and fetal brain and in lymphoblastoid cells. |
Additional Images
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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES14446-50
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