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eEF2K (phospho Ser366) rabbit pAb
AO-06-ES1470-50
Antibody Product Overview
| ELK.No | ES1470 |
| Product name | eEF2K (phospho Ser366) rabbit pAb |
| Reactivity | Human;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | EEF2K; Eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 29904 |
| Human Swiss-Prot | O00418 |
| Source | Rabbit |
| Isotype | IgG |
| Target | eEF2K |
| Fields | >>AMPK signaling pathway;>>Oxytocin signaling pathway |
| Gene name | EEF2K |
| Protein name | Eukaryotic elongation factor 2 kinase |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | O08796 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25435 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P70531 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human eEF2K around the phosphorylation site of Ser366. AA range:331-380 |
| Specificity | Phospho-eEF2K (S366) Polyclonal Antibody detects endogenous levels of eEF2K protein only when phosphorylated at S366. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 105kD |
| Background | This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.,enzyme regulation:Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.,function:Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.,similarity:Belongs to the protein kinase superfamily. Alpha-type protein kinase family.,similarity:Contains 1 alpha-type protein kinase domain.,subunit:Monomer or homodimer ., |
| Subcellular location | cytoplasm,cytosol,postsynaptic density, |
| Expression | Epithelium,Glial tumor,Lymph,T-cell, |
Additional Images
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Western Blot analysis of various cells using Phospho-eEF2K (S366) Polyclonal Antibody diluted at 1:1000
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eEF2K (Phospho-Ser366) Antibody
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2K (Phospho-Ser366) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HeLa cells treated with serum 10% 15', using eEF2K (Phospho-Ser366) Antibody. The lane on the right is blocked with the phospho peptide.
: AO-06-ES1470-50
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