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Ets-1 (phospho Thr38) rabbit pAb
AO-06-ES1520-100
Antibody Product Overview
| ELK.No | ES1520 |
| Product name | Ets-1 (phospho Thr38) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | ETS1; EWSR2; Protein C-ets-1; p54 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 2113 |
| Human Swiss-Prot | P14921 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Ets-1 |
| Fields | >>Ras signaling pathway;>>Cellular senescence;>>Human T-cell leukemia virus 1 infection;>>Pathways in cancer;>>Renal cell carcinoma |
| Gene name | ETS1 |
| Protein name | Protein C-ets-1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 23871 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P27577 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24356 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P41156 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ETS1 around the phosphorylation site of Thr38. AA range:11-60 |
| Specificity | Phospho-Ets-1 (T38) Polyclonal Antibody detects endogenous levels of Ets-1 protein only when phosphorylated at T38. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 54kD |
| Background | This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011], |
| Function | disease:ETS is responsible for erythroblast and fibroblast transformation. The juxtaposition of the interferon and c-ETS-1 proto-oncogene may be involved in the pathogenesis of human monocytic leukemia.,function:Transcription factor.,PTM:Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.,PTM:Ubiquitinated; which induces proteasomal degradation.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,similarity:Contains 1 PNT (pointed) domain.,subunit:Interacts with MAF and MAFB (By similarity). Binds to DAXX. Interacts with UBE2I., |
| Subcellular location | Cytoplasm . Nucleus . Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27. . |
| Expression | Highly expressed within lymphoid cells. Isoforms c-ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney. |
Additional Images
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Western Blot analysis of various cells using Phospho-Ets-1 (T38) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western Blot analysis of VEC cells using Phospho-Ets-1 (T38) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ETS1 (Phospho-Thr38) Antibody
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Immunofluorescence analysis of HeLa cells, using ETS1 (Phospho-Thr38) Antibody. The picture on the right is blocked with the phospho peptide.
: AO-06-ES1520-100
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