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HNF4-α (phospho Ser313) rabbit pAb
AO-06-ES1524-50
Antibody Product Overview
| ELK.No | ES1524 |
| Product name | HNF4-α (phospho Ser313) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | HNF4A; HNF4; NR2A1; TCF14; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3172 |
| Human Swiss-Prot | P41235 |
| Source | Rabbit |
| Isotype | IgG |
| Target | HNF4α |
| Fields | >>AMPK signaling pathway;>>Maturity onset diabetes of the young |
| Gene name | HNF4A |
| Protein name | Hepatocyte nuclear factor 4-alpha |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15378 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P49698 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25735 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P22449 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HNF4 alpha around the phosphorylation site of Ser313. AA range:280-329 |
| Specificity | Phospho-HNF4-α (S313) Polyclonal Antibody detects endogenous levels of HNF4-α protein only when phosphorylated at S313. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 52kD |
| Background | The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012], |
| Function | alternative products:Additional isoforms seem to exist,disease:Defects in HNF4A are the cause of maturity onset diabetes of the young type 1 (MODY1) [MIM:125850]; also shortened MODY-1. MODY [MIM:606391] is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age) and a primary defect in insulin secretion. The clinical phenotype of MODY1 is characterized by severe insulin secretory defects, and by major hyperglycemia associated with microvascular complications.,function:Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.,miscellaneous:Binds fatty acids.,online information:Hepatocyte nuclear fac |
| Subcellular location | Nucleus. |
| Expression | Kidney,Liver, |
Additional Images
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Western Blot analysis of various cells using Phospho-HNF4-α (S313) Polyclonal Antibody diluted at 1:1000
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HNF4 alpha (Phospho-Ser313) Antibody
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Immunofluorescence analysis of LOVO cells, using HNF4 alpha (Phospho-Ser313) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using HNF4 alpha (Phospho-Ser313) Antibody. The picture on the right is blocked with the phospho peptide.
: AO-06-ES1524-50
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