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Antibody Product Overview
| ELK.No | ES1589 |
| Product name | Actin rabbit pAb |
| Reactivity | Human;Mouse;Rat;Fish |
| Applications | WB;IHC;IF;ELISA |
| Other name | ACTB; Actin; cytoplasmic 1; Beta-actin; POTEKP; ACTBL3; FKSG30; Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; ACTG1; ACTB; ACTG; Actin, cytoplasmic 2; Gamma-actin |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 60/71 |
| Human Swiss-Prot | P60709/Q9BYX7/P63261 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Actin pan |
| Fields | >>Rap1 signaling pathway;>>Phagosome;>>Apoptosis;>>Hippo signaling pathway;>>Focal adhesion;>>Adherens junction;>>Tight junction;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Leukocyte transendothelial migration;>>Thermogenesis;>>Regulation of actin cytoskeleton;>>Thyroid hormone signaling pathway;>>Oxytocin signaling pathway;>>Gastric acid secretion;>>Amyotrophic lateral sclerosis;>>Bacterial invasion of epithelial cells;>>Vibrio cholerae infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Yersinia infection;>>Influenza A;>>Proteoglycans in cancer;>>Hepatocellular carcinoma;>>Hypertrophic cardiomyopathy;>>Arrhythmogenic right ventricular cardiomyopathy;>>Dilated cardiomyopathy;>>Viral myocarditis;>>Fluid shear stress and atherosclerosis |
| Gene name | ACTG1 |
| Protein name | Actin cytoplasmic 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 11461/11465 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | 81822/100361457 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P60711/P63259 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Actin. AA range:326-375 |
| Specificity | Actin Polyclonal Antibody detects endogenous levels of Actin protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 45kD |
| Background | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008], |
| Function | disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the |
| Subcellular location | Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. . |
| Expression | B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car |
Additional Images
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Western Blot analysis of various cells using Actin Polyclonal Antibody diluted at 1:2000
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Western Blot analysis of HeLa cells using Actin Polyclonal Antibody diluted at 1:2000
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Western blot analysis of various lysis using Actin Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Western blot analysis of various lysis using Actin Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
: AO-06-ES1589-100
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