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Antibody Product Overview
| ELK.No | ES1600 |
| Product name | ADAR2 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | ADARB1; ADAR2; DRADA2; RED1; Double-stranded RNA-specific editase 1; RNA-editing deaminase 1; RNA-editing enzyme 1; dsRNA adenosine deaminase |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 104 |
| Human Swiss-Prot | P78563 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ADAR2 |
| Fields | |
| Gene name | ADARB1 |
| Protein name | Double-stranded RNA-specific editase 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 110532 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q91ZS8 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25367 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P51400 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ADARB1. AA range:481-530 |
| Specificity | ADAR2 Polyclonal Antibody detects endogenous levels of ADAR2 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 80kD |
| Background | This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. [provided by RefSeq, Jul 2008], |
| Function | alternative products:Additional isoforms seem to exist,cofactor:Binds 1 inositol hexakisphosphate (IP6) per subunit.,function:Editing of the messenger RNAs for glutamate receptor (GluR) subunits by site-selective adenosine deamination. Edits both the GluR-B Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently.,similarity:Contains 1 A to I editase domain.,similarity:Contains 2 DRBM (double-stranded RNA-binding) domains., |
| Subcellular location | Nucleus . Nucleus, nucleolus . Shuttles between nucleoli and the nucleoplasm. .; [Isoform 1]: Nucleus . Nucleus, nucleolus .; [Isoform 2]: Nucleus . Nucleus, nucleolus . |
| Expression | Highly expressed in brain and heart and at lower levels in placenta. Fair expression in lung, liver and kidney. Detected in brain, heart, kidney, lung and liver (at protein level). ; [Isoform 5]: Highly expressed in hippocampus and colon. Expressed in pediatric astrocytomas and the protein has a decreased RNA-editing activity. The decrease in RNA editing correlates with the grade of malignancy of the tumors, with the high grade tumors showing lower editing is seen. |
Additional Images
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Western Blot analysis of various cells using ADAR2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of lysates from HepG2 cells, using ADARB1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES1600-100
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