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Antibody Product Overview
| ELK.No | ES16143 |
| Product name | GNAT3 rabbit pAb |
| Reactivity | Human; Mouse;Rat |
| Applications | WB |
| Other name | Guanine nucleotide-binding protein G(t) subunit alpha-3 (Gustducin alpha-3 chain) |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 346562 |
| Human Swiss-Prot | A8MTJ3 |
| Source | Rabbit |
| Isotype | IgG |
| Target | GNAT3 |
| Fields | >>Taste transduction;>>Carbohydrate digestion and absorption |
| Gene name | GNAT3 |
| Protein name | GNAT3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 242851 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q3V3I2 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 286924 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P29348 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from human GNAT3 AA range: 192-242 |
| Specificity | This antibody detects endogenous levels of GNAT3 at Human/Mouse/Rat |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.53% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 38kD |
| Background | Sweet, bitter, and umami tastes are transmitted from taste receptors by a specific guanine nucleotide binding protein. The protein encoded by this gene is the alpha subunit of this heterotrimeric G protein, which is found not only in the oral epithelium but also in gut tissues. Variations in this gene have been linked to metabolic syndrome. [provided by RefSeq, Dec 2015], |
| Function | function:Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonuim and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phosp |
| Subcellular location | Cytoplasm . Dual distribution pattern; plasmalemmal pattern with apical region localization and cytosolic pattern with localization throughout the cytoplasm. |
| Expression | Expressed in taste buds (sensory organs of clustered epithelial cells) of the circumvallate and foliate papillae of the tongue at protein level. Expressed in enteroendocrine L cells of the gut. Detected also in spermatozoa. |
Additional Images
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Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES16143-50
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