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Antibody Product Overview
| ELK.No | ES16670 |
| Product name | EST1 rabbit pAb |
| Reactivity | Human; Mouse |
| Applications | WB |
| Other name | Liver carboxylesterase 1 (Acyl-coenzyme A:cholesterol acyltransferase) (ACAT) (Brain carboxylesterase hBr1) (Carboxylesterase 1) (CE-1) (hCE-1) (EC 3.1.1.1) (Cocaine carboxylesterase) (Egasyn) (HMSE) (Methylumbelliferyl-acetate deacetylase 1) (EC 3.1.1.56) (Monocyte/macrophage serine esterase) (Retinyl ester hydrolase) (REH) (Serine esterase 1) (Triacylglycerol hydrolase) (TGH) |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 1066 |
| Human Swiss-Prot | P23141 |
| Source | Rabbit |
| Isotype | IgG |
| Target | EST1 |
| Fields | >>Drug metabolism - other enzymes |
| Gene name | CES1 CES2 SES1 |
| Protein name | EST1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 12623 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8VCC2 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized peptide derived from human EST1 AA range: 7-57 |
| Specificity | This antibody detects endogenous levels of EST1 at Human/Mouse |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.142% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 60kD |
| Background | This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010], |
| Function | catalytic activity:A carboxylic ester + H(2)O = an alcohol + a carboxylate.,enzyme regulation:Activated by CHAPS.,function:Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl CoA ester.,PTM:Contains sialic acid.,similarity:Belongs to the type-B carboxylesterase/lipase family.,subunit:Homotrimer and homohexamer. Binds to beta-glucuronidase.,tissue specificity:Expressed predominantly in liver with lower levels in heart and lung., |
| Subcellular location | Endoplasmic reticulum lumen . Cytoplasm . Lipid droplet . Moves from cytoplasm to lipid droplets upon lipid loading. Associates with lipid droplets independently of triglycerides (TG) content of the droplets and hydrolyzes cholesteryl esters more efficiently from mixed droplets. . |
| Expression | Expressed predominantly in liver with lower levels in heart and lung (PubMed:10562416). Expressed in macrophages (PubMed:11015575, PubMed:21049984, PubMed:18762277). |
Additional Images
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Western blot analysis of lysates from A549 cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES16670-50
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