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Antibody Product Overview
| ELK.No | ES1677 |
| Product name | APHC rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | ACER3; APHC; PHCA; Alkaline ceramidase 3; AlkCDase 3; Alkaline CDase 3; Alkaline dihydroceramidase SB89; Alkaline phytoceramidase; aPHC |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 55331 |
| Human Swiss-Prot | Q9NUN7 |
| Source | Rabbit |
| Isotype | IgG |
| Target | APHC |
| Fields | >>Sphingolipid metabolism;>>Metabolic pathways |
| Gene name | ACER3 |
| Protein name | Alkaline ceramidase 3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 66190 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9D099 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PHCA. AA range:171-220 |
| Specificity | APHC Polyclonal Antibody detects endogenous levels of APHC protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 35kD |
| Background | enzyme regulation:Activated by Ca(2+) and inhibited by Zn(2+).,function:Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.,similarity:Belongs to the alkaline ceramidase family.,tissue specificity:Ubiquitously expressed. Highest expression in placenta., |
| Function | enzyme regulation:Activated by Ca(2+) and inhibited by Zn(2+).,function:Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.,similarity:Belongs to the alkaline ceramidase family.,tissue specificity:Ubiquitously expressed. Highest expression in placenta., |
| Subcellular location | Endoplasmic reticulum membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . |
| Expression | Ubiquitously expressed. Highly expressed in placenta (PubMed:11356846). Expressed in erythrocytes (PubMed:20207939). |
Additional Images
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Western Blot analysis of various cells using APHC Polyclonal Antibody
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Immunofluorescence analysis of MCF7 cells, using PHCA Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using PHCA Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COLO cells, using PHCA Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES1677-100
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