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Antibody Product Overview
| ELK.No | ES17025 |
| Product name | DAD1 rabbit pAb |
| Reactivity | Human; Mouse;Rat |
| Applications | WB |
| Other name | |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 1603 |
| Human Swiss-Prot | P61803 |
| Source | Rabbit |
| Isotype | IgG |
| Target | DAD1 |
| Fields | >>N-Glycan biosynthesis;>>Various types of N-glycan biosynthesis;>>Metabolic pathways;>>Protein processing in endoplasmic reticulum |
| Gene name | DAD1 |
| Protein name | DAD1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 13135 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P61804 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 192275 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P61805 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from human DAD1 AA range: 38-88 |
| Specificity | This antibody detects endogenous levels of DAD1 at Human/Mouse/Rat |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1：500-2000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 12kD |
| Observed band (KD) | |
| Background | DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.,function:Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.,similarity:Belongs to the DAD/OST2 family.,subunit:Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, |
| Subcellular location | Endoplasmic reticulum membrane; Multi-pass membrane protein . |
| Expression |
Additional Images
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Western blot analysis of lysates from MDA-MB cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES17025-100
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