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Antibody Product Overview
| ELK.No | ES1706 |
| Product name | ARP-1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | NR2F2; ARP1; TFCOUP2; COUP transcription factor 2; COUP-TF2; Apolipoprotein A-I regulatory protein 1; ARP-1; COUP transcription factor II; COUP-TF II; Nuclear receptor subfamily 2 group F member 2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 7026 |
| Human Swiss-Prot | P24468 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ARP-1 |
| Fields | |
| Gene name | NR2F2 |
| Protein name | COUP transcription factor 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 11819 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P43135 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 113984 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | O09018 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human COT2. AA range:1-50 |
| Specificity | ARP-1 Polyclonal Antibody detects endogenous levels of ARP-1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 45kD |
| Background | This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010], |
| Function | function:Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A.,similarity:Belongs to the nuclear hormone receptor family.,similarity:Belongs to the nuclear hormone receptor family. NR2 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Interacts with SQSTM1 (By similarity). Binds DNA as a homodimer.,tissue specificity:Found in all tissues., |
| Subcellular location | Nucleus. |
| Expression | Ubiquitous. Expressed in the stromal cells of developing fetal ovaries (PubMed:29478779). |
Additional Images
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Western Blot analysis of various cells using ARP-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of HUVEC cells, using COT2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HUVEC cells, using COT2 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES1706-50
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