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Antibody Product Overview
| ELK.No | ES1847 |
| Product name | Casein Kinase Iα rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 1452 |
| Human Swiss-Prot | P48729 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Casein Kinase Iα |
| Fields | >>Wnt signaling pathway;>>Hedgehog signaling pathway;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Human papillomavirus infection;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer |
| Gene name | CSNK1A1 |
| Protein name | Casein kinase I isoform alpha |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q8BK63 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 113927 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P97633 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CKI-alpha. AA range:126-175 |
| Specificity | Casein Kinase Iα Polyclonal Antibody detects endogenous levels of Casein Kinase Iα protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 35kD |
| Background | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 on 'Ser-45'.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer. Interacts with the Axin complex., |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 on 'Ser-45'.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer. Interacts with the Axin complex., |
| Subcellular location | Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Chromosome, centromere, kinetochore . Nucleus speckle . Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm, cytoskeleton, spindle . Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. Also recruited to the keratin cytoskeleton (PubMed:23902688). . |
| Expression | Brain,Epithelium,Liver,Lung,Placenta,Skin, |
Additional Images
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Western Blot analysis of various cells using Casein Kinase Iα Polyclonal Antibody
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Immunofluorescence analysis of HeLa cells, using CKI-alpha Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CKI-alpha Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HeLa cells, using CKI-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES1847-50
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