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GAL4 Activation Domain rabbit pAb
AO-06-ES20744-100
Antibody Product Overview
| ELK.No | ES20744 |
| Product name | GAL4 Activation Domain rabbit pAb |
| Reactivity | |
| Applications | WB |
| Other name | YM3410 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | |
| Human Swiss-Prot | |
| Source | Rabbit |
| Isotype | IgG |
| Target | GAL4 Activation Domain |
| Fields | |
| Gene name | |
| Protein name | |
| Human gene link | |
| Human Swiss link | |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Recombinant Protein of GAL4 Activation Domain |
| Specificity | The antibody detects endogenous GAL4 Activation Domain protein. |
| Formulation | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Clonality | Polyclonal |
| Dilution | WB: 1:10000-50000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | |
| Background | The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators. Since the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation , this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (aa 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells. A unique "two-hybrid" system has been developed using GAL4 fusions in yeast to identify specific protein-protein interactions. |
| Function | |
| Subcellular location | |
| Expression |
Additional Images
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Western blot analysis of Recombinant AD protein using GAL4 Activation Domain Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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: AO-06-ES20744-100
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