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Antibody Product Overview
| ELK.No | ES20756 |
| Product name | EYFP rabbit pAb |
| Reactivity | Species independent |
| Applications | WB |
| Other name | YM3229 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | |
| Human Swiss-Prot | |
| Source | Rabbit |
| Isotype | IgG |
| Target | EYFP |
| Fields | |
| Gene name | |
| Protein name | |
| Human gene link | |
| Human Swiss link | |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Recombinant Protein of EYFP |
| Specificity | The antibody detects EYFP and YFP tag fusion proteins. |
| Formulation | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Clonality | Polyclonal |
| Dilution | WB: 1:3000-5000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Concentration | |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | |
| Background | Enhanced Yellow florescent protein.Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore. |
| Function | |
| Subcellular location | |
| Expression |
Additional Images
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Western blot analysis of EYFP recombinant protein, diluted at 1:5000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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: AO-06-ES20756-50
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