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Antibody Product Overview
| ELK.No | ES2121 |
| Product name | Cytochrome b5 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | CYB5A; CYB5; Cytochrome b5; Microsomal cytochrome b5 type A; MCB5 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 1528 |
| Human Swiss-Prot | P00167 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Cytochrome b5 |
| Fields | |
| Gene name | CYB5A |
| Protein name | Cytochrome b5 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 109672 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P56395 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 64001 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P00173 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CYB5. AA range:61-110 |
| Specificity | Cytochrome b5 Polyclonal Antibody detects endogenous levels of Cytochrome b5 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 15kD |
| Background | The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010], |
| Function | disease:Defects in CYB5A are the cause of type IV hereditary methemoglobinemia [MIM:250790].,function:Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.,similarity:Belongs to the cytochrome b5 family.,similarity:Contains 1 cytochrome b5 heme-binding domain., |
| Subcellular location | [Isoform 1]: Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Microsome membrane; Single-pass membrane protein; Cytoplasmic side.; [Isoform 2]: Cytoplasm. |
| Expression | Erythrocyte,Liver,Spleen,Uterus, |
Additional Images
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Western Blot analysis of various cells using Cytochrome b5 Polyclonal Antibody
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Immunofluorescence analysis of NIH/3T3 cells, using CYB5 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from A549 cells, using CYB5 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES2121-100
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