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Antibody Product Overview
| ELK.No | ES2245 |
| Product name | eIF3K rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA |
| Other name | EIF3K; EIF3S12; ARG134; HSPC029; MSTP001; PTD001; Eukaryotic translation initiation factor 3 subunit K; eIF3k; Eukaryotic translation initiation factor 3 subunit 12; Muscle-specific gene M9 protein; PLAC-24; eIF-3 p25; eIF-3 p28 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 27335 |
| Human Swiss-Prot | Q9UBQ5 |
| Source | Rabbit |
| Isotype | IgG |
| Target | eIF3K |
| Fields | |
| Gene name | EIF3K |
| Protein name | Eukaryotic translation initiation factor 3 subunit K |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 73830 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9DBZ5 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EIF3K. AA range:61-110 |
| Specificity | eIF3K Polyclonal Antibody detects endogenous levels of eIF3K protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 30kD |
| Background | The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM, Mar 2008], |
| Function | function:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.,mass spectrometry: PubMed:17322308,mass spectrometry: PubMed:18599441,PTM:The N-terminus is blocked.,similarity:Belongs to the eIF-3 subunit K family.,subunit:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, wh |
| Subcellular location | Nucleus . Cytoplasm . |
| Expression | Ubiquitous, with the highest levels of expression in brain, testis and kidney. |
Additional Images
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Western Blot analysis of various cells using eIF3K Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from HeLa cells, using EIF3K Antibody. The lane on the right is blocked with the synthesized peptide.
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: AO-06-ES2245-50
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