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Antibody Product Overview
| ELK.No | ES2294 |
| Product name | ERF rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | ERF; ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE-2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 2077 |
| Human Swiss-Prot | P50548 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ERF |
| Fields | |
| Gene name | ERF |
| Protein name | ETS domain-containing transcription factor ERF |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 13875 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P70459 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ERF. AA range:492-541 |
| Specificity | ERF Polyclonal Antibody detects endogenous levels of ERF protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 59kD |
| Observed band (KD) | |
| Background | ETS2 is a transcription factor and protooncogene involved in development, apoptosis, and the regulation of telomerase. The protein encoded by this gene binds to the ETS2 promoter and is a strong repressor of ETS2 transcription. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Aug 2015], |
| Function | function:Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation.,PTM:Phosphorylated by multiple kinases including probably ERK2. Phosphorylation regulates the activity of ERF.,sequence caution:The sequence differs from that shown because it seems to be derived from a pre-mRNA.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,tissue specificity:Highest levels in testis, ovary, pancreas, and heart., |
| Subcellular location | Nucleus. |
| Expression | Highest levels in testis, ovary, pancreas, and heart. |
Additional Images
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Western Blot analysis of various cells using ERF Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western Blot analysis of HepG2 cells using ERF Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunofluorescence analysis of HeLa cells, using ERF Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ERF Antibody. The picture on the right is blocked with the synthesized peptide.
: AO-06-ES2294-50
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