Français
English
Antibody Product Overview
| ELK.No | ES2329 |
| Product name | FAST-1/2 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IF;ELISA |
| Other name | FOXH1; FAST1; FAST2; Forkhead box protein H1; Forkhead activin signal transducer 1; Fast-1; hFAST-1; Forkhead activin signal transducer 2; Fast-2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 8928 |
| Human Swiss-Prot | O75593 |
| Source | Rabbit |
| Isotype | IgG |
| Target | FAST-1/2 |
| Fields | |
| Gene name | FOXH1 |
| Protein name | Forkhead box protein H1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 14106 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | O88621 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FOXH1. AA range:10-59 |
| Specificity | FAST-1/2 Polyclonal Antibody detects endogenous levels of FAST-1/2 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 40kD |
| Background | FOXH1 encodes a human homolog of Xenopus forkhead activin signal transducer-1. FOXH1 protein binds SMAD2 and activates an activin response element via binding the DNA motif TGT(G/T)(T/G)ATT. [provided by RefSeq, Jul 2008], |
| Function | domain:The FM region is required for binding smad2/smad4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated smad2 that is in an activated smad complex.,function:Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).,similarity:Contains 1 fork-head DNA-binding domain.,subunit:Interacts with the MH2 domains of SMAD2 and SMAD3.,tissue specificity:Ubiquitous., |
| Subcellular location | Nucleus. |
| Expression | Ubiquitous. |
Additional Images
No image
Western Blot analysis of various cells using FAST-1/2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
No image
Immunofluorescence analysis of A549 cells, using FOXH1 Antibody. The picture on the right is blocked with the synthesized peptide.
No image
No image
: AO-06-ES2329-50
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package