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Antibody Product Overview
| ELK.No | ES2372 |
| Product name | Frizzled-3 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IF;ELISA |
| Other name | FZD3; Frizzled-3; Fz-3; hFz3 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 7976 |
| Human Swiss-Prot | Q9NPG1 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Frizzled-3 |
| Fields | >>mTOR signaling pathway;>>Wnt signaling pathway;>>Axon guidance;>>Hippo signaling pathway;>>Signaling pathways regulating pluripotency of stem cells;>>Melanogenesis;>>Cushing syndrome;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Human papillomavirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>MicroRNAs in cancer;>>Basal cell carcinoma;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer |
| Gene name | FZD3 |
| Protein name | Frizzled-3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 14365 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q61086 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FZD3. AA range:141-190 |
| Specificity | Frizzled-3 Polyclonal Antibody detects endogenous levels of Frizzled-3 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 76kD |
| Background | frizzled class receptor 3(FZD3) Homo sapiens This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010], |
| Function | domain:Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.,domain:The FZ domain is involved in binding with Wnt ligands.,function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphog |
| Subcellular location | Membrane; Multi-pass membrane protein. Cell membrane ; Multi-pass membrane protein . Cell surface . Apical cell membrane ; Multi-pass membrane protein. Colocalizes with FZD6 at the apical face of the cell (By similarity). . |
| Expression | Widely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis. |
Additional Images
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Western Blot analysis of various cells using Frizzled-3 Polyclonal Antibody
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Western Blot analysis of RAW264.7 cells using Frizzled-3 Polyclonal Antibody
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Immunofluorescence analysis of COS7 cells, using FZD3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from A549 and RAW264.7 cells, using FZD3 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES2372-100
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