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Antibody Product Overview
| ELK.No | ES2414 |
| Product name | GCSc-γ rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | GCLC; GLCL; GLCLC; Glutamate--cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 2729 |
| Human Swiss-Prot | P48506 |
| Source | Rabbit |
| Isotype | IgG |
| Target | GCSc-γ |
| Fields | >>Cysteine and methionine metabolism;>>Glutathione metabolism;>>Metabolic pathways;>>Biosynthesis of cofactors;>>Ferroptosis |
| Gene name | GCLC |
| Protein name | Glutamate--cysteine ligase catalytic subunit |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 14629 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P97494 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25283 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P19468 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GCSc-gamma. AA range:266-315 |
| Specificity | GCSc-γ Polyclonal Antibody detects endogenous levels of GCSc-γ protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 73kD |
| Background | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010], |
| Function | catalytic activity:ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.,disease:Defects in GCLC are the cause of hemolytic anemia [MIM:230450].,enzyme regulation:Feedback inhibition by glutathione.,pathway:Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.,similarity:Belongs to the glutamate--cysteine ligase type 3 family.,subunit:Heterodimer of a catalytic heavy chain and a regulatory light chain., |
| Subcellular location | cytosol,integral component of membrane,glutamate-cysteine ligase complex, |
| Expression | Foreskin fibroblast,Hippocampus,Liver,Testis,Thalamus, |
Additional Images
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Western Blot analysis of various cells using GCSc-γ Polyclonal Antibody
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Western blot analysis of lysate from A549 cells, using GCSc-γ antibody.
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: AO-06-ES2414-50
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