Français
English
Antibody Product Overview
| ELK.No | ES2428 |
| Product name | GK1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IF;ELISA |
| Other name | GK; Glycerol kinase; GK; Glycerokinase; ATP:glycerol 3-phosphotransferase |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 2710 |
| Human Swiss-Prot | P32189 |
| Source | Rabbit |
| Isotype | IgG |
| Target | GK1 |
| Fields | >>Glycerolipid metabolism;>>Metabolic pathways;>>PPAR signaling pathway |
| Gene name | GK |
| Protein name | Glycerol kinase |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 14933 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q64516 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 79223 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q63060 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GK. AA range:461-510 |
| Specificity | GK1 Polyclonal Antibody detects endogenous levels of GK1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 57kD |
| Background | The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011], |
| Function | catalytic activity:ATP + glycerol = ADP + sn-glycerol 3-phosphate.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,disease:Defects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.,function:Key enzyme in the regulation of glycerol uptake and metabolism.,pathway:Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.,similarity:Belongs to the FGGY kinase family.,subcellular location:In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.,tissue specificity:Highly expressed in the liver, kidney and testis |
| Subcellular location | Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm. |
| Expression | Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver. |
Additional Images
No image
Western Blot analysis of various cells using GK1 Polyclonal Antibody diluted at 1:2000
No image
Immunofluorescence analysis of HeLa cells, using GK Antibody. The picture on the right is blocked with the synthesized peptide.
No image
Western blot analysis of lysates from 293 and Jurkat cells, using GK Antibody. The lane on the right is blocked with the synthesized peptide.
No image
: AO-06-ES2428-50
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package