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Antibody Product Overview
| ELK.No | ES2564 |
| Product name | HPK1 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | MAP4K1; HPK1; Mitogen-activated protein kinase kinase kinase kinase 1; Hematopoietic progenitor kinase; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 11184 |
| Human Swiss-Prot | Q92918 |
| Source | Rabbit |
| Isotype | IgG |
| Target | HPK1 |
| Fields | >>MAPK signaling pathway |
| Gene name | MAP4K1 |
| Protein name | Mitogen-activated protein kinase kinase kinase kinase 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 26411 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P70218 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MEKKK 1. AA range:371-420 |
| Specificity | HPK1 Polyclonal Antibody detects endogenous levels of HPK1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 91kD |
| Background | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 CNH domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with MAP3K1.,tissue specificity:Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine., |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 CNH domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with MAP3K1.,tissue specificity:Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine., |
| Subcellular location | intracellular,cytoplasm,membrane, |
| Expression | Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. |
Additional Images
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Western Blot analysis of various cells using HPK1 Polyclonal Antibody diluted at 1:1000
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from HepG2 cells, using MEKKK 1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HUVECcells using MEKKK 1 antibody.
: AO-06-ES2564-100
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