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Antibody Product Overview
| ELK.No | ES2584 |
| Product name | HSP60 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | HSPD1; HSP60; 60 kDa heat shock protein; mitochondrial; 60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 3329 |
| Human Swiss-Prot | P10809 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Hsp60 |
| Fields | >>RNA degradation;>>Type I diabetes mellitus;>>Legionellosis;>>Tuberculosis;>>Lipid and atherosclerosis |
| Gene name | HSPD1 |
| Protein name | 60 kDa heat shock protein mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15510 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P63038 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 63868 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P63039 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSP60. AA range:511-560 |
| Specificity | HSP60 Polyclonal Antibody detects endogenous levels of HSP60 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 68kD |
| Background | This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010], |
| Function | disease:Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.,disease:Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first 2 decades of life.,function:Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the |
| Subcellular location | Mitochondrion matrix. |
| Expression | Adipocyte,Adrenal gland,B-cell lymphoma,Brain,Cajal-Retzius |
Additional Images
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Immunofluorescence analysis of Hela cell. 1,HSP60 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
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Western Blot analysis of various cells using HSP60 Polyclonal Antibody diluted at 1:2000
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Western Blot analysis of COLO205 cells using HSP60 Polyclonal Antibody diluted at 1:2000
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
: AO-06-ES2584-100
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