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Antibody Product Overview
| ELK.No | ES2591 |
| Product name | Id4 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | ID4; BHLHB27; DNA-binding protein inhibitor ID-4; Class B basic helix-loop-helix protein 27; bHLHb27; Inhibitor of DNA binding 4 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3400 |
| Human Swiss-Prot | P47928 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Id4 |
| Fields | >>TGF-beta signaling pathway;>>Signaling pathways regulating pluripotency of stem cells |
| Gene name | ID4 |
| Protein name | DNA-binding protein inhibitor ID-4 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15904 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P41139 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ID4. AA range:1-50 |
| Specificity | Id4 Polyclonal Antibody detects endogenous levels of Id4 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 24kD |
| Background | This gene encodes a member of the inhibitor of DNA binding (ID) protein family. These proteins are basic helix-loop-helix transcription factors which can act as tumor suppressors but lack DNA binding activity. Consequently, the activity of the encoded protein depends on the protein binding partner. [provided by RefSeq, Dec 2011], |
| Function | function:ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Heterodimer with other HLH proteins., |
| Subcellular location | Nucleus. |
| Expression | Abdominal adipose tissue,Uterus, |
Additional Images
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Western Blot analysis of various cells using Id4 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from HepG2 cells, using ID4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using ID4 antibody.
: AO-06-ES2591-50
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