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Antibody Product Overview
| ELK.No | ES2681 |
| Product name | Ku-70 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | XRCC6; G22P1; X-ray repair cross-complementing protein 6; 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box- |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 2547 |
| Human Swiss-Prot | P12956 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Ku70/XRCC6 |
| Fields | >>Non-homologous end-joining |
| Gene name | XRCC6 |
| Protein name | X-ray repair cross-complementing protein 6 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 14375 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P23475 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Ku70. AA range:560-609 |
| Specificity | Ku-70 Polyclonal Antibody detects endogenous levels of Ku-70 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 70kD |
| Background | The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008], |
| Function | developmental stage:Expression does not increase during promyelocyte differentiation.,disease:Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to p70 and p86. Existence of a major autoantigenic epitope or epitopes on the C-terminal 190 amino acids of p70 containing the leucine repeat. The majority of autoantibodies to p70 in most sera from patients with SLE seem to be reactive with this region.,function:Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 |
| Subcellular location | Nucleus . Chromosome . |
| Expression | Brain,Cervix carcinoma,Epithelium,Heart,Hepatocyte,Kidney,Liver,Lun |
Additional Images
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Western Blot analysis of various cells using Ku-70 Polyclonal Antibody
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Immunofluorescence analysis of COS7 cells, using Ku70 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Ku70 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HepG2 cells, using Ku70 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES2681-100
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