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Antibody Product Overview
| ELK.No | ES2749 |
| Product name | MAST205 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | MAST2; KIAA0807; MAST205; Microtubule-associated serine/threonine-protein kinase 2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 23139 |
| Human Swiss-Prot | Q6P0Q8 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MAST205 |
| Fields | |
| Gene name | MAST2 |
| Protein name | Microtubule-associated serine/threonine-protein kinase 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 17776 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q60592 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MAST2. AA range:1201-1250 |
| Specificity | MAST205 Polyclonal Antibody detects endogenous levels of MAST205 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 196kD |
| Background | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation.,PTM:Phosphorylated and ubiquitinated. N-terminal ubiquitination leads to degradation of MAST2 by proteasome-mediated proteolysis. N-terminal phosphorylation appears to be a prerequisite for ubiquitination.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Recruited to the sub-membranous area on interaction with PC-LKC.,tissue specificity:Abundant in the testis., |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation.,PTM:Phosphorylated and ubiquitinated. N-terminal ubiquitination leads to degradation of MAST2 by proteasome-mediated proteolysis. N-terminal phosphorylation appears to be a prerequisite for ubiquitination.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contain |
| Subcellular location | Cytoplasm, cytoskeleton . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Recruited to the sub-membranous area on interaction with CDHR2. |
| Expression | Abundant in the testis. |
Additional Images
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Western Blot analysis of various cells using MAST205 Polyclonal Antibody
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from Jurkat, 293, and HepG2 cells, using MAST2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from K562 cells using MAST2 antibody.
: AO-06-ES2749-50
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