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Antibody Product Overview
| ELK.No | ES2828 |
| Product name | MRP-L12 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA;IHC |
| Other name | MRPL12; RPML12; 39S ribosomal protein L12; mitochondrial; L12mt; MRP-L12; 5c5-2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 6182 |
| Human Swiss-Prot | P52815 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MRP-L12 |
| Fields | >>Ribosome |
| Gene name | MRPL12 |
| Protein name | 39S ribosomal protein L12 mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 56282 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9DB15 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPL12. AA range:31-80 |
| Specificity | MRP-L12 Polyclonal Antibody detects endogenous levels of MRP-L12 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 21kD |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq, Jul 2008], |
| Function | similarity:Belongs to the ribosomal protein L12P family., |
| Subcellular location | Mitochondrion . |
| Expression | Muscle,Tonsil, |
Additional Images
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Western Blot analysis of various cells using MRP-L12 Polyclonal Antibody
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Western blot analysis of lysates from COLO cells, using MRPL12 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES2828-50
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