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Antibody Product Overview
| ELK.No | ES2851 |
| Product name | MRP-S34 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA;IHC |
| Other name | MRPS34; 28S ribosomal protein S34; mitochondrial; MRP-S34; S34mt |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 65993 |
| Human Swiss-Prot | P82930 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MRP-S34 |
| Fields | |
| Gene name | MRPS34 |
| Protein name | 28S ribosomal protein S34 mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 79044 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9JIK9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPS34. AA range:169-218 |
| Specificity | MRP-S34 Polyclonal Antibody detects endogenous levels of MRP-S34 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 26kD |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014], |
| Function | subunit:Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins., |
| Subcellular location | Mitochondrion . |
| Expression | Eye, |
Additional Images
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Western Blot analysis of various cells using MRP-S34 Polyclonal Antibody diluted at 1:1000
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Western Blot analysis of Jurkat cells using MRP-S34 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from HeLa, HUVEC, HT-29, A549, and Jurkat cells, using MRPS34 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES2851-50
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