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Antibody Product Overview
| ELK.No | ES2858 |
| Product name | Sin3B rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA;IHC |
| Other name | SIN3B; KIAA0700; Paired amphipathic helix protein Sin3b; Histone deacetylase complex subunit Sin3b; Transcriptional corepressor Sin3b |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 23309 |
| Human Swiss-Prot | O75182 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Sin3B |
| Fields | |
| Gene name | SIN3B |
| Protein name | Paired amphipathic helix protein Sin3b |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q62141 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SIN3B. AA range:221-270 |
| Specificity | Sin3B Polyclonal Antibody detects endogenous levels of Sin3B protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 130kD |
| Background | function:Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription.,similarity:Contains 3 PAH (paired amphipathic helix) repeats.,subunit:Interacts with FOXK1/MNF, MXI, MAD, NCOR1 and SAP30. Interaction with SDS3 enhances the interaction with HDAC1 to form a complex. Interacts with MAD3, MAD4, MAEL, REST and SETDB1 (By similarity). Interacts with HCFC1., |
| Function | function:Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription.,similarity:Contains 3 PAH (paired amphipathic helix) repeats.,subunit:Interacts with FOXK1/MNF, MXI, MAD, NCOR1 and SAP30. Interaction with SDS3 enhances the interaction with HDAC1 to form a complex. Interacts with MAD3, MAD4, MAEL, REST and SETDB1 (By similarity). Interacts with HCFC1., |
| Subcellular location | Nucleus . |
| Expression | Brain,Duodenum,Lung,Skin, |
Additional Images
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Western Blot analysis of various cells using Sin3B Polyclonal Antibody
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Immunofluorescence analysis of HeLa cells, using SIN3B Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from A549 cells, using SIN3B Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES2858-50
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