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Antibody Product Overview
| ELK.No | ES2878 |
| Product name | Myogenin rabbit pAb |
| Reactivity | Human;Mouse;Rat;Pig(Test by out customer);Pig |
| Applications | WB;IHC;IF;ELISA |
| Other name | MYOG; BHLHC3; MYF4; Myogenin; Class C basic helix-loop-helix protein 3; bHLHc3; Myogenic factor 4; Myf-4 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 4656 |
| Human Swiss-Prot | P15173 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Myogenin |
| Fields | |
| Gene name | MYOG |
| Protein name | Myogenin |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 17928 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P12979 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 29148 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P20428 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Myogenin. AA range:50-99 |
| Specificity | Myogenin Polyclonal Antibody detects endogenous levels of Myogenin protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 39kD |
| Background | Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008], |
| Function | function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein., |
| Subcellular location | Nucleus. Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin-remodeling and transcription initiation in developing embryos. . |
| Expression | Eye,Skeletal muscle, |
Additional Images
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Western Blot analysis of various cells using Myogenin Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunohistochemical analysis of paraffin-embedded Human liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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Immunohistochemical analysis of paraffin-embedded Human liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
No image
Immunohistochemical analysis of paraffin-embedded Human liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
: AO-06-ES2878-100
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