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Antibody Product Overview
| ELK.No | ES2914 |
| Product name | NEEP21 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | NSG1; D4S234; Neuron-specific protein family member 1; Brain neuron cytoplasmic protein 1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 27065 |
| Human Swiss-Prot | P42857 |
| Source | Rabbit |
| Isotype | IgG |
| Target | NEEP21 |
| Fields | |
| Gene name | NSG1 |
| Protein name | Neuron-specific protein family member 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 18196 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q62092 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25247 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P02683 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NSG1. AA range:112-161 |
| Specificity | NEEP21 Polyclonal Antibody detects endogenous levels of NEEP21 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 26kD |
| Background | similarity:Belongs to the NSG family., |
| Function | similarity:Belongs to the NSG family., |
| Subcellular location | Membrane ; Single-pass type II membrane protein . Golgi apparatus, trans-Golgi network membrane . Endosome membrane . Cell projection, dendrite . Early endosome membrane . Late endosome membrane . Lysosome lumen . Recycling endosome membrane . Cytoplasmic vesicle membrane . Golgi apparatus, Golgi stack membrane . Endosome, multivesicular body membrane . Endoplasmic reticulum membrane . Endocytosed from the cell surface, thus enters into early endosomes, trafficks to late endosomes and degradates in lysosomes (By similarity). Endoplasmic reticulum targeting is essential for apoptosis (PubMed:20599942). Found in both stationary and motile endosomes. A previous study supports a type I membrane protein topology (By similarity). . |
| Expression | Amygdala,Brain,Cerebellum,Testis, |
Additional Images
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Western Blot analysis of various cells using NEEP21 Polyclonal Antibody diluted at 1:2000
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from COLO cells, using NSG1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from K562 cells using NSG1 antibody.
: AO-06-ES2914-50
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