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Antibody Product Overview
| ELK.No | ES3001 |
| Product name | Oct-2 rabbit pAb |
| Reactivity | Human;Mouse;Monkey |
| Applications | WB;IHC;IF;ELISA |
| Other name | POU2F2; OCT2; OTF2; POU domain; class 2, transcription factor 2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Octamer-binding protein 2; Oct-2; Octamer-binding transcription factor 2; OTF-2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 5452 |
| Human Swiss-Prot | P09086 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Oct-2 |
| Fields | >>Herpes simplex virus 1 infection;>>Lipid and atherosclerosis |
| Gene name | POU2F2 |
| Protein name | POU domain class 2 transcription factor 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 18987 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q00196 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human OCT2. AA range:1-50 |
| Specificity | Oct-2 Polyclonal Antibody detects endogenous levels of 40818 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 52kD |
| Background | The protein encoded by this gene is a homeobox-containing transcription factor of the POU domain family. The encoded protein binds the octamer sequence 5'-ATTTGCAT-3', a common transcription factor binding site in immunoglobulin gene promoters. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011], |
| Function | function:Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.,similarity:Belongs to the POU transcription factor family. Class-2 subfamily.,similarity:Contains 1 homeobox DNA-binding domain.,similarity:Contains 1 POU-specific domain.,subunit:Interacts with NR3C1, AR and PGR.,tissue specificity:Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line Molt-4, but not in the T-cell line BW 5147., |
| Subcellular location | Cytoplasm . Nucleus. |
| Expression | Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147. |
Additional Images
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Western Blot analysis of various cells using Oct-2 Polyclonal Antibody
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Immunofluorescence analysis of COS7 cells, using OCT2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COS7 cells, using OCT2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES3001-50
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